MST2 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10559

应用：

WB,IHC-P,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

56 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

KRS1; KRS2; MST 1; MST1; MST 2; MST2; STE20 like kinase MST1; STE20 like kinase MST2; STK3; STK 3; STK4; STK 4; Serine/threonine kinase 3; Serine/threonine kinase 4; Serine/threonine protein kinase 3; Serine/threonine protein kinase 4

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:5000

IHC-P 1:50-1:200

IP Use at an assay dependent concentration.

Immunogen

Information

Gene Name：

STK3

Protein Name：

Serine/threonine-protein kinase 3

Gene ID：

6788 (Human)

56274 (Mouse)

65189 (Rat)

SwissPro：

Q13188 (Human)

Q9JI10 (Mouse)

O54748 (Rat)

Subcellular Location：

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Immunogen：

Synthetic peptide within N-terminal human MST2.

Specificity:

MST2 Monoclonal Antibody detects endogenous levels of MST2 protein.

Product images
	Fig : Western blot analysis of MST2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10559, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell Lane 2: LN229 cell Lane 3: U251 cell Lane 4: 4T1 cell Lane 5: RAW264.7 cell Lane 6: HEPA1-6 cell Lane 7: SHZ-88 cell Lane 8: RBL-2H3 cell Predicted molecular weight:56KD Observed molecular weight:63KD，40KD (Serine/threonine-protein kinase 3 36kDa subunit) Exposure time: 420 sec
	Fig : Immunohistochemical analysis of paraffin-embedded RM-1 cell derived xenograft tissue with Rabbit anti-MST2 antibody (AWA10559) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10559) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.