NF2 Recombinant Rabbit Monoclonal Antibody

货号:
AWA10557
应用:
WB,IF-C,FCM
反应性:
Human,Mouse,Rat,Monkey
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat,Monkey

    Molecular Wt:

    70 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    ACN; BANF; Merlin; merlin-1; Schwannomerlin; Schwannomin; Neurofibromin 2; Neurofibromin-2; Neurofibromin2; Neurofibromatosis type 2; Neurofibromatosis2; neurofibromin 2 (merlin); NF2; NF 2; SCH; Moesin ezrin radixin like protein


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IF-C 1:50-1:200
    FCM 1:1000



    Immunogen

    Information

    Gene Name:

    NF2

    Protein Name:

    Merlin


    Gene ID:

    4771 (Human)    
    18016 (Mouse)    
    25744 (Rat)

    SwissPro:

    P35240 (Human)    
    P46662 (Mouse)    
    Q63648 (Rat)


    Subcellular Location:

    Cell projection, filopodium membrane. Cell projection, ruffle membrane. Nucleus. Cytoplasm, perinuclear region. Cytoplasmic granule. Cytoplasm, cytoskeleton.


    Immunogen:

    Recombinant protein within human NF2. AA range: 1-350.


    Specificity:

    NF2 Monoclonal Antibody detects endogenous levels of NF2 protein.




    Product images
    NF2 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of NF2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10557, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: 4T1 cell
    Lane 2: HT22 cell
    Lane 3: Rat brain tissue
    Lane 4: SHZ-88 cell

    Predicted molecular weight:70KD
    Observed molecular weight:72-75KD

    Exposure time:90 sec
    NF2 Recombinant Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of NF2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10557, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell
    Lane 2: MCF-7 cell
    Lane 3: Jurkat cell
    Lane 4: PC-3 cell
    Lane 5: HepG2 cell
    Lane 6: SH-SY5Y cell
    Lane 7: A431 cell
    Lane 8: LN229 cell
    Lane 9: NIH3T3 cell
    Lane 10: GL261 cell

    Predicted molecular weight:70KD
    Observed molecular weight:72-75KD

    Exposure time:90 sec
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