MYL2 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10550

应用：

WB,IHC-P,IP

反应性：

Human,Mouse,Rat,Zebrafish

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat,Zebrafish

Molecular Wt:

19 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

MLC 2; MLC 2v; MLC2; MLC2V; MYL2; Myosin Light Chain 2; Myosin Light Chain 2/MLC-2V

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:5000

IHC-P 1:50-1:200

IP Use at an assay dependent concentration.

Immunogen

Information

Gene Name：

MYL2

Protein Name：

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform

Gene ID：

4633 (Human)

17906 (Mouse)

363925 (Rat)

SwissPro：

P10916 (Human)

P51667 (Mouse)

P08733 (Rat)

Subcellular Location：

Cytoplasm.

Immunogen：

Synthetic peptide within Human MYL2 . AA range: 117-166.

Specificity:

MYL2 Monoclonal Antibody detects endogenous levels of MYL2 protein.

Product images
	Fig: Fluorescence immunohistochemical analysis of Rat-muscle tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-MYL2 antibody (AWA10550) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10550) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Western blot analysis of MYL2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10550, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat heart tissue Predicted molecular weight:19KD Observed molecular weight:19KD Exposure time:45 sec