DNMT3A Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10549

应用：

WB,IHC-P,IF-C,IF-T

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

102 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; DNMT3A; DNMT 3a; Dnmt3a; DNMT; DNMT3A2; M.HsaIIIA; MCMT; TBRS

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:5000

IHC-P 1:200

IF-C 1:50-1:200

IF-T 1:50-1:200

Immunogen

Information

Gene Name：

DNMT3A

Protein Name：

DNA (cytosine-5)-methyltransferase 3A

Gene ID：

1788 (Human)

13435 (Mouse)

444984 (Rat)

SwissPro：

Q9Y6K1 (Human)

O88508 (Mouse)

Q1LZ53 (Rat)

Subcellular Location：

Nucleus. Chromosome. Cytoplasm.

Immunogen：

Synthetic peptide within human DNMT3A. AA range: 600-640.

Specificity:

DNMT3A Monoclonal Antibody detects endogenous levels of DNMT3A protein.

Product images
	Fig : Western blot analysis of DNMT3A on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10549, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell Lane 2: HEK-293 cell Lane 3: HepG2 cell Lane 4: Hela cell Lane 5: NIH3T3 cell Lane 6: HEPA1-6 cell Lane 7: Rat brain tissue Lane 8: Rat heart tissue Predicted molecular weight:102 kDa Observed molecular weight:100-110,140 kDa Exposure time:180 sec
	Fig: Immunocytochemistry analysis of Hela cells labeling DNMT3A with Rabbit anti-DNMT3A antibody (AWA10549) at 1/50 dilution(green ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated withRabbit anti-DNMT3A antibody (AWA10549) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	AWA10549
	Fig: Fluorescence immunohistochemical analysis of Rat-spleen tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-DNMT3A antibody (AWA10549) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10549) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.