IRE1(Phospho Ser724) Recombinant Rabbit Monoclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details
| Host Species: Rabbit | Reactivity: Human,Mouse | Molecular Wt: 110 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1 mg/ml | ||
Other Names: ERN1; hIRE1p; Inositol requiring protein 1; IRE1; Ire1 alpha; IRE1; ERN1; IRE1P; IRE1α
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Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: -20°C,1 year | ||||
Applications
| WB 1:1000 IHC-P 1:500 IF 1:500 FCM 1:1000
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Immunogen Information | Gene Name: ERN1 | Protein Name: Serine/threonine-protein kinase/endoribonuclease IRE1
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Gene ID: 2081 (Human) 78943 (Mouse)
| SwissPro: O75460 (Human) Q9EQY0 (Mouse)
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Subcellular Location: Endoplasmic reticulum membrane, Nucleus. | ||||
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser724 of human IRE1.
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Specificity: Phospho IRE1 (Ser724) Monoclonal Antibody detects endogenous levels of Phospho IRE1 (Ser724) protein.
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Product images | |
Fig : Western blot analysis of IRE1(Phospho Ser724) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA10545, 1/1000) was used in TBST(0.3%TWEEN20) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.(10% SDS-PAGE gel.) Positive control: Lane 1: K562 cell lysate Lane 2: HL60 cell lysate Lane 3: Jurkat cell lysate Lane 4: Hela cell lysate Lane 5: PC12 cell lysate Lane 6: A549 cell lysate Lane 7: PC-3 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: C2C12 cell lysate Lane 10: HepG2 cell lysate Predicted molecular weight: 109.8KD Observed molecular weight: 130KD Exposure time: 180 seconds |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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