IRE1(Phospho Ser724) Recombinant Rabbit Monoclonal Antibody

货号:
AWA10545
应用:
WB,IHC-P,IF,FCM
反应性:
Human,Mouse
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse

    Molecular Wt:

    110 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    ERN1; hIRE1p; Inositol requiring protein 1; IRE1; Ire1 alpha; IRE1; ERN1; IRE1P; IRE1α

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:1000

    IHC-P 1:500

    IF 1:500

    FCM 1:1000

     



    Immunogen

    Information

    Gene Name:

    ERN1 

    Protein Name:

    Serine/threonine-protein kinase/endoribonuclease IRE1

     


    Gene ID:

    2081 (Human)     

    78943 (Mouse)     

     

    SwissPro:

    O75460 (Human)     

    Q9EQY0 (Mouse)     

     


    Subcellular Location:

    Endoplasmic reticulum membrane, Nucleus.


    Immunogen:

    Synthetic phospho-peptide corresponding to residues surrounding Ser724 of human IRE1.

     


    Specificity:

    Phospho IRE1 (Ser724) Monoclonal Antibody detects endogenous levels of Phospho IRE1 (Ser724) protein.

     




    Product images
    IRE1(Phospho Ser724) Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of IRE1(Phospho Ser724) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA10545, 1/1000) was used in TBST(0.3%TWEEN20) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.(10% SDS-PAGE gel.)
    Positive control:
    Lane 1: K562 cell lysate
    Lane 2: HL60 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: Hela cell lysate
    Lane 5: PC12 cell lysate
    Lane 6: A549 cell lysate
    Lane 7: PC-3 cell lysate
    Lane 8: Mouse brain tissue lysate
    Lane 9: C2C12 cell lysate
    Lane 10: HepG2 cell lysate

    Predicted molecular weight: 109.8KD
    Observed molecular weight: 130KD

    Exposure time: 180 seconds
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