NR2F1 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10544

应用：

WB,IHC-P

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

46 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

COUP TF I; COUP TF1; COUP TFI; COUP transcription factor 1; COUP transcription factor I; EAR 3; EAR3; ERBAL3; NR2F1; NR2F2; SVP44; TCFCOUP1; TFCOUP1; V erbA related protein 3

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000

IHC-P 1:1000

Immunogen

Information

Gene Name：

NR2F1

Protein Name：

COUP transcription factor 1

Gene ID：

7025 (Human)

13865 (Mouse)

81808 (Rat)

SwissPro：

P10589 (Human)

Q60632 (Mouse)

Q62681 (Rat)

Subcellular Location：

Nucleus.

Immunogen：

Synthetic peptide within Human NR2F1. AA range: 1-50.

Specificity:

NR2F1 Monoclonal Antibody detects endogenous levels of NR2F1 protein.

Product images
	Fig: Fluorescence immunohistochemical analysis of Mouse-cerebellum tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-NR2F1 antibody (AWA10544) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10544) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-brain tissue with Rabbit anti-NR2F1 antibody (AWA10544) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10544) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of NR2F1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10544, 1/2000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain Lane 2: PC12 cell Lane 3: Hela cell Lane 4: NIH3T3 cell Predicted molecular weight:46 kDa Observed molecular weight:44 kDa; Exposure time: 90 seconds
	Fig: Fluorescence immunohistochemical analysis of Mouse-retina tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-NR2F1 antibody (AWA10544) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10544) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.