GYS1 Recombinant Rabbit Monoclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 84 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: glycogen synthase 1 (muscle); Glycogen synthase 1; muscle; GSY; GYS; GYS1 | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name： GYS1 | Protein Name： Glycogen [starch] synthase, muscle | ||
Gene ID： 2997 (Human) | SwissPro： P13807 (Human) | |||
Subcellular Location： Cytoplasm. Cytosol. Inclusion body. Membrane. | ||||
Immunogen： Recombinant protein within human GYS1. AA range: 638-737. | ||||
Specificity: GYS1 Monoclonal Antibody detects endogenous levels of GYS1 protein. | ||||
| Product images | |
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Fig : Western blot analysis of GYS1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA10543, 1/1000) was used in TBST(0.3%TWEEN20) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.(10% SDS-PAGE gel.) Positive control: Lane 1: HEK293 cell Lane 2: A431 cell Predicted molecular weight: 84 kDa Observed molecular weight: 84 kDa Exposure time: 15 seconds |
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Fig : Western blot analysis of GYS1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA10543, 1/1000) was used in TBST(0.3%TWEEN20) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.(10% SDS-PAGE gel.) Positive control: Lane 1: Hela cell lysate Lane 2: NRK-49F cell lysate Lane 3: RM-1 cell lysate Lane 4: HepG2 cell lysate Lane 5: HSC-T6 cell lysate Lane 6: MCF-7 cell lysate Lane 7: SHZ-88 cell lysate Predicted molecular weight: 84 kDa Observed molecular weight: 84 kDa Exposure time: 90 seconds |
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Fig: Immunocytochemistry analysis of A431 cells labeling GYS1 with Rabbit anti-GYS1 antibody (AWA10543) at 1/50 dilution(green ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-GYS1 antibody (AWA10543) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
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Fig: Fluorescence immunohistochemical analysis of Mouse-Heart muscle tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-GYS1 antibody (AWA10543) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10543) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
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Fig : Immunohistochemical analysis of paraffin-embedded Mouse-heart muscle tissue with Rabbit anti-GYS1 antibody (AWA10543) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10543) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig: Fluorescence immunohistochemical analysis of Rat-Muscle tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-GYS1 antibody (AWA10543) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10543) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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