MYH1 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10538

应用：

IHC-P,IF

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

223 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

MYH1; MYHa; MyHC 2x; MyHC 2X/D; MyHC IIx/d; MYHSA1; Myosin 1; Myosin heavy chain 1; Myosin heavy chain 2x; Myosin heavy chain IIx/d

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

IHC-P 1:2000-1:10000

IF 1:400-1:2000

Immunogen

Information

Gene Name：

MYH1

Protein Name：

Myosin-1

Gene ID：

4619 (Human)

17879 (Mouse)

SwissPro：

P12882 (Human)

Q5SX40 (Mouse)

Subcellular Location：

Cytoplasm, myofibril.

Immunogen：

Synthetic peptide within human MYH1.

Specificity:

MYH1 Monoclonal Antibody detects endogenous levels of MYH1 protein.

Product images
	Fig: Fluorescence immunohistochemical analysis of Rat-muscle tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-MYH1 antibody (AWA10538) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10538) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-heart muscle tissue with Rabbit anti-MYH1 antibody (AWA10538) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10538) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX
	Fig: Immunocytochemistry analysis of U20S cells labeling MYH1 with Rabbit anti-MYH1 antibody (AWA10538) at 1/50 dilution(green ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-MYH1 antibody (AWA10538) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).