P2RY12 Recombinant Rabbit Monoclonal Antibody

货号:
AWA10535
应用:
IHC-P,IHC-F,IF-T
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    39 kDa


    Clonality:

    Monoclonal 

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    ADP glucose receptor; ADP-glucose receptor; ADPG R; ADPG-R; ADPGR; HORK 3; HORK3; P2RY 12; P2RY12; P2T(AC); P2Y purinoceptor 12, P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y(12)R; P2Y 12 ; P2Y12; P2Y12_HUMAN; P2Y12 platelet ADP receptor; SP1999; SP 1999; BDPLT8; Platelet ADP receptor

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    IHC-P 1:200-1:1000

    IHC-F 1:200

    IF-T 1:200

     



    Immunogen

    Information

    Gene Name:

    P2RY12

    Protein Name:

    P2Y purinoceptor 12


    Gene ID:

    64805 (Human)     

    70839 (Mouse)     

    64803 (Rat)

     

    SwissPro:

    Q9H244 (Human)     

    Q9CPV9 (Mouse)     

    Q9EPX4 (Rat)

     


    Subcellular Location:

    Cell membrane.


    Immunogen:

    Synthetic peptide.


    Specificity:

    P2RY12 Monoclonal Antibody detects endogenous levels of P2RY12 protein.


    Product images
    P2RY12 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-hippocampus tissue with Rabbit anti-P2RY12 antibody (AWA10535) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10535) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    P2RY12 Recombinant Rabbit Monoclonal Antibody - 2 Fig : Immunohistochemical analysis of paraffin-embedded Rat-hippocampus tissue with Rabbit anti-P2RY12 antibody (AWA10535) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10535) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    P2RY12 Recombinant Rabbit Monoclonal Antibody - 3 Fig: Fluorescence immunohistochemical analysis of Mouse-Cerebellum tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-P2Y12 antibody (AWA10535) at 1/200 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10535) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
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