Smad3 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10524

应用：

WB,IHC-P,IF-C,IF-T,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

48 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:5000

IHC-P 1:50-1:200

IF-C 1:50-1:200

IF-T 1:50-1:200

FCM 1:50-1:100

Immunogen

Information

Gene Name：

SMAD3

Protein Name：

Mothers against decapentaplegic homolog 3

Gene ID：

4088 (Human)

17127 (Mouse)

25631 (Rat)

SwissPro：

P84022 (Human)

Q8BUN5 (Mouse)

P84025 (Rat)

Subcellular Location：

Cytoplasm. Nucleus.

Immunogen：

Synthetic peptide within human Smad3. AA range: 181-230.

Specificity:

Smad3 Monoclonal Antibody detects endogenous levels of Smad3 protein.

Product images
	Fig : Western blot analysis of Smad3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10524, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HT-29 cell Lane 2: 4T1 cell Predicted molecular weight:48kDa Observed molecular weight:55 kDa Exposure time：45s
	Fig : Western blot analysis of Smad3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10524, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell Lane 2: HT1080 cell Lane 3: K562 cell Lane 4: Mouse spleen Lane 5: PC12 cell Predicted molecular weight:48kDa Observed molecular weight:55 kDa Exposure time：3min
	Fig: Immunocytochemistry analysis of A549 cells labeling Smad3 with rabbit anti-Smad3 antibody (AWA10524) at 1/50 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with rabbit anti-Smad3 antibody (AWA10524) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).