CD3 Recombinant Rabbit Monoclonal Antibody

货号:
AWA10523
应用:
WB,IHC-P,IF-C,IF-T,mIHC,IP
反应性:
Human
来源:
Rabbit
  • 20μL 
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human

    Molecular Wt:

    23 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG 

    Concentration:

    1 mg/ml


    Other Names:

    CD3; CD3 epsilon; CD3-epsilon; CD3E; CD3E_HUMAN; T3E; TCRE; CD3E antigen epsilon polypeptide; CD3E antigen, epsilon subunit; CD3e molecule epsilon; IMD18; T3E

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:1000-1:2000

    IHC-P 1:200-1:600

    IF-C 1:100

    IF-T 1:100-1:200

    mIHC 1:500

    IP Use at an assay dependent concentration.

     



    Immunogen

    Information

    Gene Name:

    CD3E

    Protein Name:

    T-cell surface glycoprotein CD3 epsilon chain


    Gene ID:

    916 (Human)     

    SwissPro:

    P07766 (Human)     


    Subcellular Location:

    Cell membrane.


    Immunogen:

    Synthetic peptide within human CD3E. AA range: 158-207.


    Specificity:

    CD3 Monoclonal Antibody detects endogenous levels of CD3 protein.


    Product images
    CD3 Recombinant Rabbit Monoclonal  Antibody - 1 Fig : Western blot analysis of CD3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA10523, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Jurkat cell lysate

    Predicted molecular weight: 23KD
    Observed molecular weight: 24KD
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