IκB-α Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10522

应用：

WB,IHC-P,IF-C,IF-T,FCM,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

36 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

I kappa B alpha; I-kappa-B-alpha; IkappaBalpha; IkB alpha; IkB-alpha; IKBA; IKBA_HUMAN; MAD 3; MAD3; NF kappa B inhibitor alpha; NF-kappa-B inhibitor alpha; NFKBI; NFKBIA

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:5000

IHC-P 1:50-1:200

IF-C 1:50-1:200

IF-T 1:50-1:200

FCM 1:1000

IP Use at an assay dependent concentration.

Immunogen

Information

Gene Name：

NFKBIA

Protein Name：

NF-kappa-B inhibitor alpha

Gene ID：

4792 (Human)

18035 (Mouse)

25493 (Rat)

SwissPro：

P25963 (Human)

Q9Z1E3 (Mouse)

Q63746 (Rat)

Subcellular Location：

Cytoplasm. Nucleus.

Immunogen：

Synthetic peptide within human IKB alpha. AA range: 1-50.

Specificity:

IκB-α Monoclonal Antibody detects endogenous levels of IκB-α protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti-IIκB-α (AWA10522) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10522) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Rabbit anti-IκB-α (AWA10522) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10522) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Fluorescence immunohistochemical analysis of Rat-testis tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-IκB-α antibody (AWA10522) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10522) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.