Smad3(Phospho Ser423/425) Recombinant Rabbit Monoclonal Antibody

货号：

AWA10521

应用：

WB,IHC-P,IF-C,IF-T

反应性：

Human,Mouse,Rat,Monkey

来源：

Rabbit

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat,Monkey

Molecular Wt:

48 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

LDS3; mad3; LDS1C; MADH3; JV15-2; hMAD-3; hSMAD3; HSPC193; HsT17436; Phospho-Smad3-S423/S425

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:5000

IHC-P 1:50-1:500

IF-C 1:50-1:200

IF-T 1:50-1:200

Immunogen

Information

Gene Name：

SMAD3

Protein Name：

Mothers against decapentaplegic homolog 3

Gene ID：

4088 (Human)

17127 (Mouse)

25631 (Rat)

SwissPro：

P84022 (Human)

Q8BUN5 (Mouse)

P84025 (Rat)

Subcellular Location：

Cytoplasm. Nucleus.

Immunogen：

Synthetic phospho peptide corresponding to residues surrounding Ser423 and 425 of human Smad3.

Specificity:

Phospho Smad3 (Ser423/425) Monoclonal Antibody detects endogenous levels of Phospho Smad3 (Ser423/425) protein.

Product images
	Fig: Immunocytochemistry analysis of A549 cells (treated with 5ng/ml TGF-ß1 for 24 hours) labeling Smad3(Phospho Ser423/425) with rabbit anti-Smad3(Phospho Ser423/425) antibody (AWA10521) at 1/50 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with rabbit anti-Smad3(Phospho Ser423/425) antibody (AWA10521) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Western blot analysis of Smad3(Phospho Ser423/S425) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10521, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH3T3 cell Lane 2: MC38 cell Lane 3: C2CL2 cell Lane 4: A549 cell (A549 treated with 5ng/ml TGF-ß1 for 24 hours whole cell lysates) Lane 5: Rat heart Lane 6: A549 cell Lane 7: COS7 cell Exposure time: 15 seconds Predicted band size: 48 kDa Observed band size: 55 kDa
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-testis tissue with Rabbit anti-Smad3(Phospho Ser423/425) (AWA10521) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10521) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-Smad3(Phospho Ser423/425) (AWA10521) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10521) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue with Rabbit anti-Smad3(Phospho Ser423/425) (AWA10521) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10521) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-cerebellum tissue with Rabbit anti-Smad3(Phospho Ser423/425) (AWA10521) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10521) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Rabbit anti-Smad3(Phospho Ser423/425) (AWA10521) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10521) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-large intestine tissue with Rabbit anti-Smad3(Phospho Ser423/425) (AWA10521) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10521) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-lung tissue with Rabbit anti-Smad3(Phospho Ser423/425) (AWA10521) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10521) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.