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- 20μL
- ￥620
- 1-3个工作日
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- 50μL
- ￥1250
- 1-3个工作日
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- 100μL
- ￥2200
- 1-3个工作日
Product Details
| Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 34 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1 mg/ml | ||
Other Names: CCND1; BCL1; PRAD1; G1/S-specific cyclin-D1; G1/S specific cyclin D1; B-cell lymphoma 1 protein; B cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene; cyclin D; cyclin D1; U21B31
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Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: -20°C,1 year | ||||
Applications
| WB 1:1000-1:5000 IHC-P 1:200-1:1000 IF-C 1:50-1:200 IF-T 1:50-1:200 FCM 1:5000 IP Use at an assay dependent concentration.
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Immunogen Information | Gene Name： CCND1 | Protein Name： G1/S-specific cyclin-D1 | ||
Gene ID： 595 (Human) 12443 (Mouse) 58919 (Rat) | SwissPro： P24385 (Human) P25322 (Mouse) P39948 (Rat)
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Subcellular Location： Nucleus. Cytoplasm. Nucleus membrane. | ||||
Immunogen： Synthetic peptide within C-terminal human Cyclin D1. | ||||
Specificity: Cyclin D1 Monoclonal Antibody detects endogenous levels of Cyclin D1 protein. | ||||
| Product images | |
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Fig : Immunohistochemical analysis of paraffin-embedded mouse-lung tissue with Rabbit anti-Cyclin D1 antibody (AWA10518) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10518) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-lung tissue with Rabbit anti-Cyclin D1 antibody (AWA10518) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10518) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Western blot analysis of Cyclin D1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10518, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: N-2A cell Lane 2: NIH/3T3 cell Lane 3: 4T1 cell Lane 4: SHZ-88cell Lane 5: PC12 cell Predicted molecular weight:34 kDa Observed molecular weight:36 KDa Exposure time: 15 seconds |
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Fig : Immunohistochemical analysis of paraffin-embedded Mouse-Kidney tissue with Rabbit anti-Cyclin D1 antibody (AWA10518) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10518) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-stomach tissue with Rabbit anti-Cyclin D1 antibody (AWA10518) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10518) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-Cyclin D1 antibody (AWA10518) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10518) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
-
-
- 20μL
- ￥620
- 1-3个工作日
-
- 50μL
- ￥1250
- 1-3个工作日
-
- 100μL
- ￥2200
- 1-3个工作日
-
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