Ubiquitin(linkage-specific K63) Recombinant Rabbit Monoclonal Antibody

货号：

AWA10460

应用：

WB,IHC-P,IF-C,IF-T

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

15,18,26,77 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

Epididymis secretory protein Li 50; FLJ25987; HEL S 50; MGC8385; Polyubiquitin B; RPS 27A; RPS27A; UBA 52; UBA 80; UBA52; UBA80; UBB; UBB_HUMAN; UBC; UBCEP 1; UBCEP 2; UBCEP1; UBCEP2; Ubiquitin B; K63-linkageSpecificUbiquitin; K63linkageSpecificUbiquitin; Ubiquitin

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000

IHC-P 1:50-1:200

IF-C 1:100-1:500

IF-T 1:100-1:500

Immunogen

Information

Gene Name：

UBB

Protein Name：

Polyubiquitin-B

Gene ID：

7314/7316/6233/7311 (Human)

22187 (Mouse)

192255 (Rat)

SwissPro：

P0CG47/P0CG48/P62979/P62987 (Human)

P0CG49/P62991 (Mouse)

P0CG51/P62989 (Rat)

Subcellular Location：

Cytoplasm. Nucleus. Mitochondrion outer membrane.

Immunogen：

Synthetic peptide within Human K63-linkage Specific Ubiquitin. AA range: 36-85.

Specificity:

Ubiquitin (linkage-specific K63) Monoclonal Antibody detects endogenous levels of Ubiquitin (linkage-specific K63) protein.

Product images
	Fig : Western blot analysis of Ubiquitin(linkage-specific K63) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10460, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HEK293 cell Lane 2: Hela cell Lane 3: MCF-7 cell Lane 4: HepG2 cell Lane 5: PC3 cell Lane 6: N-2A cell Lane 7: NIH3T3 cell Predicted molecular weight:26KD Observed molecular weight:28-180KD Exposure time:180 sec
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-Ubiquitin(linkage-specific K63) antibody (AWA10460) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10460) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-brain tissue with Rabbit anti-Ubiquitin(linkage-specific K63) antibody (AWA10460) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10460) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.