PSMD14 Recombinant Rabbit Monoclonal Antibody

货号:
AWA10455
应用:
WB,IHC-P
反应性:
Human,Rat,Mouse,Pig,Monkey
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Rat,Mouse,Pig,Monkey

    Molecular Wt:

    35 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    PAD1; POH1; PSMD14; PSMD14/POH1; rpn11; RPN11; 26S proteasome-associated PAD1 homolog 1; PSMD14


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IHC-P 1:50-1:200



    Immunogen

    Information

    Gene Name:

    PSMD14

    Protein Name:

    26S proteasome non-ATPase regulatory subunit 14


    Gene ID:

    10213 (Human)    
    59029 (Mouse)    
    311078 (Rat)

    SwissPro:

    O00487 (Human)    
    O35593 (Mouse)    
    Q4V8E2 (Rat)


    Subcellular Location:

    Cytosol. Cytosolic proteasome complex. Extracellular region. Nucleoplasm. Nucleus.


    Immunogen:

    Recombinant protein within human PSMD14. AA range: 92-230.


    Specificity:

    PSMD14 Monoclonal Antibody detects endogenous levels of PSMD14 protein.




    Product images
    PSMD14 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of PSMD14 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA10455, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HT-1080 cell
    Lane 2: NIH/3T3 cell
    Lane 3: MCF-7 cell
    Lane 4: SHZ-88 cell
    Lane 5: 4T1 cell
    Lane 6: RAW264.7 cell
    Lane 7: 3D4/21 cell
    Lane 8: K562 cell
    Lane 9: Jurkat cell
    Lane 10: Hela cell

    Predicted molecular weight: 35 kDa
    Observed molecular weight: 35 kDa
    Exposure time: 7 seconds
    PSMD14 Recombinant Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of PSMD14 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA10455, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: SK-OV-3 cell
    Lane 2: ID8 cell
    Lane 3: HEK293 cell
    Lane 4: COS7 cell
    Lane 5: PC12 cell
    Lane 6: HepG2 cell
    Lane 7: A431 cell
    Lane 8: U20S cell

    Predicted molecular weight: 35 kDa
    Observed molecular weight: 35 kDa
    Exposure time: 7 seconds
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