GAD67/GAD1 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10450

应用：

WB,IHC,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

67 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

FLJ45882; GAD; GAD 67; GAD-67; GAD1; GAD67; Glutamate decarboxylase 1; SCP; CPSQ1; DCE1; DCE1_HUMAN; Glutamate decarboxylase 1 brain 67kD

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:1000

IHC 1:50-1:200

IP 1:50-1:100

Immunogen

Information

Gene Name：

GAD1

Protein Name：

Glutamate decarboxylase 1

Gene ID：

2571 (Human)

14415 (Mouse)

24379 (Rat)

SwissPro：

Q99259 (Human)

P48318 (Mouse)

P18088 (Rat)

Subcellular Location：

Axon terminus. Cell cortex. Clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane. Cytoplasm. GABA-ergic synapse. Inhibitory synapse. Plasma membrane. Presynaptic active zone. Vesicle membrane.

Immunogen：

Synthetic peptide within Human GAD67. AA range: 60-99.

Specificity:

GAD67/GAD1 Monoclonal Antibody detects endogenous levels of GAD67/GAD1 protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-cerebellum tissue with Rabbit anti-GAD67 (AWA10450) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10450) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of GAD67 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10450, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC-12 cell Lane 2: U87-MG cell Lane 3: NPK-49F cell Lane 4: HT-22 cell Lane 5: N-2A cell Lane 6: U251 cell Lane 7: Rat brain tissue Predicted band size: 67 kDa Observed band size: 67 kDa