Annexin I Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10446

应用：

WB,IHC,IF,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1周
50μL ¥1250 1周
100μL ¥2200 1周

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

39 KDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

ANXA1; ANX1; LPC1; Annexin A1; Annexin I; Annexin 1; Annexin-1; Calpactin II; Calpactin 2; Calpactin-2; Chromobindin 9; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC 1:50-1:100

IF 1:50-1:200

IP 1:20

Immunogen

Information

Gene Name：

ANXA1

Protein Name：

Annexin A1

Gene ID：

301 (Human)

16952 (Mouse)

25380 (Rat)

SwissPro：

P04083 (Human)

P10107 (Mouse)

P07150 (Rat)

Subcellular Location：

Nucleus. Cytoplasm. Cell projection, cilium. Cell membrane. Membrane. Endosome membrane. Basolateral cell membrane. Apical cell membrane. Lateral cell membrane. Secreted. Secreted,extracellular space. Cell membrane Secreted,extracellular exosome. Cytoplasmic vesicle, secretory vesicle lumen. Cell projection, phagocytic cup. Early endosome. Cytoplasmic vesicle membrane.

Immunogen：

Recombinant protein of human Annexin A1.

Specificity:

Annexin I Monoclonal Antibody detects endogenous levels of Annexin I protein.

Product images
	Fig: Immunocytochemistry analysis of A431 cells labeling c-Kit with Rabbit anti-Annexin I antibody (AWA10446) at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti-c-Kit antibody (AWA10446) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti- Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Western blot analysis of Annexin I on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10446, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell Lane 2: NIH3T3 cell Lane 3: A2058 cell Lane 4: HELA cell Lane 5: 4T1 cell Lane 6: HCT116 cell Lane 7: K562 cell Lane 8: Jurkat cell Predicted band size: 39 kDa Observed band size: 37 kDa
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti-Annexin Ⅰ (AWA10446) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10446) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Rabbit anti-Annexin Ⅰ (AWA10446) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10446) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.