LY75 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10444

应用：

WB,IHC-P,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

198 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

LY75; CD205; CD 205; CD205 antigen; CLEC13B; CLEC 13B; Lymphocyte antigen 75; Ly-75; LY 75; C-type lectin domain family 13 member B; DEC-205; DEC 205; DEC205; gp200-MR6; gp200 MR6; CD antigen CD205; gp200

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:1000

IHC-P 1:50-1:200

IP Use at an assay dependent concentration.

Immunogen

Information

Gene Name：

LY75

Protein Name：

Lymphocyte antigen 75

Gene ID：

4065 (Human)

17076 (Mouse)

499800 (Rat)

SwissPro：

O60449 (Human)

Q60767 (Mouse)

D3ZQK8 (Rat)

Subcellular Location：

Membrane.

Immunogen：

Recombinant protein within Human LY75. AA range: 1560-1722.

Specificity:

LY75 Monoclonal Antibody detects endogenous levels of LY75 protein.

Product images
	Fig : Western blot analysis of LY75 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10444, 1/1500) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell Lane 2: THP-1 cell Lane 3: MOLM-13 cell Predicted molecular weight:198KD Observed molecular weight:~250KD
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti-LY75 antibody (AWA10444) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10444) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-LY75 antibody (AWA10444) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10444) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.