Smad2 Recombinant Rabbit Monoclonal Antibody

货号:
AWA10406
应用:
WB,IHC-P,IF,FCM,IP
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 有库存
  • 50μL
  • ¥1250
  • 有库存
  • 100μL
  • ¥2200
  • 有库存
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    52 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:1000-1:5000

    IHC-P 1:50-1:200

    IF 1:50-1:200

    FCM 1:50-1:100

    IP Use at an assay dependent concentration.

     



    Immunogen

    Information

    Gene Name:

    SMAD2

    Protein Name:

    Mothers against decapentaplegic homolog 2


    Gene ID:

    4087 (Human)     

    17126 (Mouse)     

    29357 (Rat)

     

    SwissPro:

    Q15796 (Human)     

    Q62432 (Mouse)     

    O70436 (Rat)

     


    Subcellular Location:

    Cytoplasm, Nucleus.


    Immunogen:

    Synthetic peptide within human Smad2. AA range: 220-270.


    Specificity:

    Smad2 Monoclonal Antibody detects endogenous levels of Smad2 protein.




    Product images
    Smad2 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of Smad2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10406, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Jurkat cell
    Lane 2: HT1080 cell
    Lane 3: Hela cell
    Lane 4: NIH3T3 cell
    Lane 5: Raw264.7 cell
    Lane 6: RBL-2H3 cell
    Lane 7: PC12 cell

    Predicted molecular weight:52 kDa
    Observed molecular weight:58 kDa
    Exposure time:3min
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