Vimentin Recombinant Rabbit Monoclonal Antibody

货号：

AWA10146

应用：

WB,IHC-P,IHC-F,IF-C,IF-T,IP,mIHC,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

Predicted MW: 54 kDa Observed MW: 54 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1mg/ml

Other Names:

VIM; CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; Vimentin

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC-F 1:100-1:1000
IF-C 1:50-1:200
IF-T 1:100-1:1000
IP 1-2ug/sample
mIHC 1:100-1:1000
FCM 1:50-1:100

Immunogen
Information

Gene Name:

VIM

Protein Name:

Vimentin

Gene ID:

7431 (Human)
22352 (Mouse)
81818 (Rat)

SwissPro:

P08670 (Human)
P20152 (Mouse)
P31000 (Rat)

Subcellular Location:

Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix. Cell membrane.

Immunogen:

Synthetic peptide within C-terminal human Vimentin.

Specificity:

Vimentin Monoclonal Antibody detects endogenous levels of Vimentin protein.

Product images
	Fig : Western blot analysis of Vimentin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10146, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell Lane 2: PC-3 cell Lane 3: LN229 cell Lane 4: HELA cell Lane 5: Jurkat cell Lane 6: NRK-49F cell Lane 7: RBL-2H3 cell Predicted molecular weight:54 kDa Observed molecular weight:54 kDa Exposure time：3 sec
	Fig: Immunocytochemistry analysis of HELA cells labeling Vimentin with Rabbit anti-Vimentin antibody (AWA10146) at 1/50 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Vimentin antibody (AWA10146) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig: Multiplex immunohistochemistry analysis of Rat-kidney tissue (Formalin/PFA-fixed paraffin-embedded sections). Merged staining of anti-LDHA (AWA10478; green; TSA-520:AWI0688), anti-vimentin (AWA10146; red; TSA-570: AWI0689), anti-AQP4 (AWA10201; white; TSA-690: AWI0691). Antibody Wash Solution (for mIHC) (AWI0707, 37℃, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. The section was incubated in three rounds of staining; in the order of LDHA (AWA10478) (1/200 dilution), anti-vimentin (AWA10146) (1/400 dilution), anti-AQP4 (AWA10201) (1/200 dilution); each using a separate fluorescent tyramide signal amplification system. DAPI (blue, AWC0291) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual TSA dyes was performed using a pannoramic midi. This image was generated from the hybridoma version.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue with Rabbit anti-vimentin (AWA10146) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10146) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of Vimentin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10146, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HEK293 cell Lane 2: Jurkat cell Lane 3: A549 cell Lane 4: NIH/3T3 cell Predicted molecular weight:54KD Observed molecular weight:54KD
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue with Rabbit anti-Vimentin (AWA10146) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10146) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-cerebellum tissue with Rabbit anti-Vimentin (AWA10146) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10146) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-heart tissue with Rabbit anti-vimentin (AWA10146) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10146) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-myocardium tissue with Rabbit anti-Vimentin (AWA10146) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10146) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-testis tissue with Rabbit anti-Vimentin (AWA10146) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10146) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.