15 Lipoxygenase 2 Rabbit Polyclonal Antibody

货号:
AWA59636
应用:
WB,IF,ELISA
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    76 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    15-LOX-2; 15 LOX 2; ALOX15B; 15 Lipoxygenase 2; 15 LOX B; ALOX8; Arachidonate 15 lipoxygenase B


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IF 1:50-1:200
    ELISA 1:20000



    Immunogen

    Information

    Gene Name:

    ALOX15B

    Protein Name:

    Polyunsaturated fatty acid lipoxygenase ALOX15B


    Gene ID:

    247 (Human)    
    266604 (Rat)

    SwissPro:

    O15296 (Human)  
    Q8K4F2 (Rat)


    Subcellular Location:

    Cytoplasm, cytosol. Cell membrane. Cytoplasm, cytoskeleton. Membrane. Cell junction, adherens junction. Cell junction, focal adhesion. Nucleus.


    Immunogen:

    Recombinant protein within human 15 Lipoxygenase 2. AA range: 1-200.


    Specificity:

    15 Lipoxygenase 2 Polyclonal Antibody detects endogenous levels of 15 Lipoxygenase 2 protein.




    Product images
    15 Lipoxygenase 2 Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of 15 Lipoxygenase 2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA59636, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: MCF-7 cell
    Lane 2: Hela cell
    Lane 3: HEK-293 cell

    Predicted molecular weight:76 kDa
    Observed molecular weight:67 kDa
    Exposure time:90seconds
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
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