GPX4 Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA59145

应用：

WB,IF

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

22 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

Glutathione peroxidase 4; GPX 4; GPX-4; GPX4; GPX4_HUMAN; GSHPx-4; MCSP; mitochondrial; PHGPx; Phospholipid hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase; snGPx; Phospholipid hydroperoxide glutathione peroxidase mitochondrial; snPHGPx; Sperm nucleus glutathione peroxidase

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:1000

IF 1:50-1:200

Immunogen

Information

Gene Name：

GPX4

Protein Name：

Phospholipid hydroperoxide glutathione peroxidase GPX4

Gene ID：

2879 (Human)

625249 (Mouse)

29328 (Rat)

SwissPro：

P36969 (Human)

O70325 (Mouse)

P36970 (Rat)

Subcellular Location：

Mitochondrion. Cytoplasm.

Immunogen：

Recombinant protein within human GPX4. AA range: 30-197.

Specificity:

GPX4 Polyclonal Antibody detects endogenous levels of GPX4 protein.

Product images
	Fig: Immunocytochemistry analysis of U2OS cells labeling GPX4 with Rabbit anti-GPX4 antibody (AWA59145) at 1/50 dilution(green ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-GPX4 antibody (AWA59145) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-GPX4 antibody (AWA59145) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA59145) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue with Rabbit anti-GPX4 antibody (AWA59145) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA59145) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.