ASC/TMS1 Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA58244

应用：

WB,IHC-p,IF,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

22 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

PYCARD; ASC; CARD5; TMS1; TMS-1; TMS; Apoptosis-associated speck-like protein containing a CARD; hASC; Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1; CARD5; ASC/TMS1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20 C. Stable for one year after shipment. Aliquoting is unnecessary for -20 C storage.

Applications

WB 1:500-1:2000

IHC-P 1:50-1:200

IF 1:50-1:200

IP Use at an assay dependent concentration.

Immunogen

Information

Gene Name：

PYCARD

Protein Name：

Apoptosis-associated speck-like protein containing a CARD

Gene ID：

29108 (Human)

66824 (Mouse)

282817 (Rat)

SwissPro：

Q9ULZ3 (Human)

Q9EPB4 (Mouse)

Q8CHK8 (Rat)

Subcellular Location：

Cytoplasm. Inflammasome. Endoplasmic reticulum. Mitochondrion, Nucleus.

Immunogen：

Recombinant protein within human ASC/TMS1. AA range: 1-91.

Specificity:

ASC/TMS1 Polyclonal Antibody detects endogenous levels of ASC/TMS1 protein.

Product images
	Fig : Western blot analysis of ASC/TMS1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA58244, 1/2000) was used in TBST at room temperature for 2 hours. Goat Anti-Ribbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell Lane 2:J774A.1 cell Lane 3: Mouse lung Lane 4:Mouse spleen Predicted molecular weight:22KD Observed molecular weight:25KD; Exposure time: 15seconds
	Fig: Fluorescence immunohistochemical analysis of mouse-Lewis cell derived xenograft tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-ASC antibody (AWA58244) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA58244) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti-ASC/TMS1 antibody (AWA58244) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA58244) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.