GSDMD Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA58029

应用：

WB,IHC,IF

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

53 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

Gasdermin-D; C-terminal; Gasdermin-D; gasderminD; Gasdermin domain-containing protein 1; gasdermin D; DF5L; DFNA5L; FKSG10; GSDMDC1; GSDMD

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:5000

IHC-P 1:50-1:300

IF 1:50-1:200

Immunogen

Information

Gene Name：

GSDMD

Protein Name：

Gasdermin-D

Gene ID：

79792 (Human)

69146 (Mouse)

SwissPro：

P57764 (Human)

Q9D8T2 (Mouse)

Subcellular Location：

Cytoplasm, cytosol. Inflammasome. Cell membrane. Secreted. Mitochondrion membrane.

Immunogen：

Synthesized peptide derived from human GSDMD. AA range: 1-100.

Specificity:

GSDMD Polyclonal Antibody detects endogenous levels of GSDMD protein.

Product images
	Fig : Western blot analysis of GSDMD on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA58029, 1/4000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH3T3 cell Lane 2: Jurkat cell Lane 3: Rat brain Lane 4: A431 cell Lane 5: PC12 cell Lane 6: Raw264.7 cell Exposure time: 15 seconds Predicted band size: 53 kDa Observed band size: 53 kDa
	Fig : Western blot analysis of GSDMD on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA58029, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC12 cell Lane 2: THP-1 cell (THP-1 treated with 80 nM TPA overnight and then 100 ng/ml LPS for 6 h and 300 ng/ml Brefeldin A for the last 3 h) Lane 3: Raw264.7 cell Lane 4: NIH3T3 cell Lane 5: THP-1 cell Exposure time: 3 seconds Predicted molecular weight:53KD Observed molecular weight:53KD(GSDMD); 43KD(Cleaved GSDMD p40 );
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-breast tissue with Rabbit anti-GSDMD (AWA58029) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA58029) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Rabbit anti-GSDMD (AWA58029) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA58029) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-lung tissue with Rabbit anti-GSDMD (AWA58029) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA58029) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Immunocytochemistry analysis of Raw264.7 cells labeling GSDMD with Rabbit anti- GSDMD antibody （AWA58029）at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti- GSDMD antibody （AWA58029）at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).