IL-1β Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA46641

应用：

WB,IHC-P,IF-C,IF-T,ELISA

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

31 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

IL1B; IL1F2; Interleukin-1 beta; IL-1 beta; Catabolin

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC-P 1:100-300

IF-C 1:100-1:300

IF-T 1:100-1:300

ELISA 1:20000

Immunogen

Information

Gene Name：

IL1B

Protein Name：

Interleukin-1 beta

Gene ID：

3553 (Human)

16176 (Mouse)

24494 (Rat)

SwissPro：

P01584 (Human)

P10749 (Mouse)

Q63264 (Rat)

Subcellular Location：

Extracellular exosome, Secreted, Lysosome, Cytosol.

Immunogen：

The antiserum was produced against synthesized peptide derived from the Internal region of human IL1B. AA range: 181-230.

Specificity:

IL-1β Polyclonal Antibody detects endogenous levels of IL-1β protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-colon tissue with Rabbit anti-IL-1βantibody (AWA46641) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA46641) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Rabbit anti-IL-1βantibody (AWA46641) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA46641) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of IL-1β on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA46641, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-rabbit IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1:Hela cell lysate Lane 2: Rat heart tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Rat liver tissue lysate
	Fig : Western blot analysis of IL-1β on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA46641, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell Lane 2: THP-1 cell(THP-1 treated with 80 nM TPA overnight and then 100 ng/ml LPS for 6 h and 300 ng/ml Brefeldin A for the last 3 h) Lane 3:Raw264.7 Lane 4:Raw264.7(treated with 100 ng/ml LPS for 7 hours and 300 ng/ml Brefeldin A for the last 3 hours)
	Fig: Immunocytochemistry analysis of Hela cells labeling IL-1β with Rabbit anti-IL-1β antibody （AWA46641）at 1/100 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-IL-1β antibody （AWA46641）at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig: Fluorescence immunohistochemical analysis of Rat-lung tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-IL-1β antibody (AWA46641) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA46641) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig: Fluorescence immunohistochemical analysis of Rat-Urinary bladder tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-IL-1β antibody (AWA46641) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA46641) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.