Cleaved Caspase-9 p10(D330) Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA46474

应用：

WB,IHC,IF,ELISA

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 有库存
50μL ¥1250 有库存
100μL ¥2200 有库存

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

10,46 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC 1:100-1:300

IF 1:50-1:200

ELISA 1:20000

Not yet tested in other applications.

Immunogen

Information

Gene Name：

CASP9

Protein Name：

Caspase-9

Gene ID：

842 (Human)

12371 (Mouse)

58918 (Rat)

SwissPro：

P55211 (Human)

Q8C3Q9 (Mouse)

Q9JHK1 (Rat)

Subcellular Location：

Nucleus, mitochondrion, cytosol, apoptosome.

Immunogen：

The antiserum was produced against synthesized peptide derived from human Caspase 9. AA range: 281-330.

Specificity:

Cleaved Caspase-9 p10 (D330) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-9 p10 protein resulting from cleavage adjacent to D330.

Product images
	Fig : Western blot analysis of Cleaved caspase9 p10 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA46474, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell Lane 2: Jurkat cell Lane 3: THP-1 cell Lane 4: Rat brain Lane 5: HL-60 cell Lane 6: NIH3T3 cell Lane 7: HepG2 cell Exposure time: 15 seconds Predicted molecular weight:10/46 kDa Observed molecular weight:52KD(Full length caspase9)、35/37/17/12 KDa(Cleaved caspase9);
	Fig : Western blot analysis of Cleaved Caspase9 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA46474, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Ribbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 3D4/21 cell Lane 2: 3D4/21 cell treated with 800 μmH2O2 for 24 hours Lane 3:Hela cell Lane 4:Hela cell treated with 1μM staurosporine for 24 hours Predicted molecular weight:46/35KD Observed molecular weight:51/38KD; Exposure time: 7seconds
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-brain tissue with Rabbit anti-Caspase 9 antibody (AWA46474) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA46474) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.