Cleaved Caspase-3 p12 (D175) Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA45558

应用：

WB,IHC-p,IF

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 有库存
50μL ¥1250 有库存
100μL ¥2200 有库存

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

12,35 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC-p 1:50-1:300

IF 1:50-1:300

Immunogen

Information

Gene Name：

CASP3

Protein Name：

Caspase-3

Gene ID：

836 (Human)

12367 (Mouse)

25402 (Rat)

SwissPro：

P42574 (Human)

P70677 (Mouse)

P55213 (Rat)

Subcellular Location：

Cytoplasm.

Immunogen：

The antiserum was produced against synthesized peptide derived from human Caspase 3. AA range:157-206.

Specificity:

Cleaved Caspase-3 p12(D175) Polyclonal Antibody detects endogenous levels of Cleaved Caspase-3 p12(D175) protein.

Product images
	Fig: Fluorescence immunohistochemical analysis of Mouse-kidney tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-Cleaved Caspase-3 p12(D175) antibody (AWA45558) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA45558) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Western blot analysis of Cleaved caspase3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA45558, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Ribbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell Lane 2: Raji cell treated with 10 μg/ml Cycloheximide over night and treated with 20 ng/ml TNF-α for 4 hours； Lane 3: 3D4/21 cell Lane 4: 3D4/21 cell treated with 800 μm H2O2 for 24 hours
	Fig: Immunocytochemistry analysis of Hela cells labeling Cleaved-Caspase-3 p12 (D175) with Rabbit anti-Cleaved-Caspase-3 p12 (D175) antibody （AWA45558）at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Cleaved-Caspase-3 p12 (D175) antibody （AWA45558 ）at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Western blot analysis of Cleaved-Caspase-3 p12 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA45558, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: U251 cell Lane 2: HEPG2 cell Lane 3: U87-MG cell Lane 4: Jurkat cell Lane 5: HELA cell Lane 6: N-2A cell
	Fig : Western blot analysis of Cleaved-Caspase-3 p12（D175） on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 90 minutes at room temperature. The primary antibody (AWA45558, 1/5000) was used in TBST at room temperature for 90 minutes. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain tissue lysate