Bcl-2 Rabbit Polyclonal Antibody
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat,Chicken,Pig | Molecular Wt: 26 kDa | |
Clonality: Polyclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: BCL2; Apoptosis regulator Bcl-2; Bcl-2 | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name： BCL2 | Protein Name： Apoptosis regulator Bcl-2 | ||
Gene ID： 596 (Human) | SwissPro： P10415 (Human) | |||
Subcellular Location： Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane. Cytoplasm. | ||||
Immunogen： The antiserum was produced against synthesized peptide derived from human Bcl-2. AA range: 46-95. | ||||
Specificity: Bcl-2 Polyclonal Antibody detects endogenous levels of Bcl-2 protein. | ||||
| Product images | |
|
|
Fig : Western blot analysis of Bcl-2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA43352, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell Lane 2: NIH3T3 cell Lane 3: THP-1 cell Lane 4: HL-1 cell Lane 5: N-2a cell Predicted molecular weight: 26 kDa Observed molecular weight: 26 kDa |
|
|
Fig : Western blot analysis of Bcl-2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA43352, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: N-2A cell Lane 2: Jurkat cell Lane 3: U251 cell Lane 4: THP-1 cell Lane 5: RBL-2H3 cell Lane 6: NPK-49F cell Lane 7: MC-38 cell Lane 8: NIH3T3 cell Predicted molecular weight: 26 kDa Observed molecular weight: 26 kDa |
|
|
Fig: Fluorescence immunohistochemical analysis of Rat-testicles tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-Bcl-2 antibody (AWA43352) at 1/50 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA43352) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
相关产品
-
Cdk6 Recombinant Rabbit Monoclonal Antibody
GAPDH Rabbit Polyclonal Antibody
GFAP Recombinant Mouse Monoclonal Antibody
Ki67 Rabbit Monoclonal Antibody
HMGB1 Recombinant Rabbit Monoclonal Antibody
SQSTM1/p62 Mouse Monoclonal Antibody
Bcl-2 Recombinant Rabbit Monoclonal Antibody
SOD2 Rabbit Polyclonal Antibody
