Cleaved Caspase-1 p20(D210) Rabbit Polyclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
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- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 25 kDa | |
Clonality: Polyclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: CASP 1; CASP1; Caspase 1; Caspase1; ICE; IL 1 beta converting enzyme; IL 1BC; IL1BC; IL1BCE; Interleukin 1 beta convertase; P45; Cleaved Caspase-1 p20(D210) | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name： CASP1 | Protein Name： Caspase-1 | ||
Gene ID： 834 (Human) | SwissPro： P29466 (Human) | |||
Subcellular Location： Cytoplasm. Cell membrane. | ||||
Immunogen： The antiserum was produced against synthesized peptide derived from human Caspase-1. AA range: 155-218. | ||||
Specificity: Cleaved Caspase-1(D210) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-1 protein. resulting from cleavage adjacent to D210. | ||||
| Product images | |
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Fig: Immunocytochemistry analysis of Hela cells labeling Cleaved-caspase1（D210）with Rabbit anti-Cleaved-caspase1（D210）antibody (AWA40005) at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Cleaved-caspase1（D210） antibody (AWA40005) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0003) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
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Fig : Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue with Rabbit anti-Casepase 1 (AWA40005) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA40005) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig: Immunocytochemistry analysis of RAW264.7 cells labeling Cleaved Caspase-1 P20 with Rabbit anti-Cleaved Caspase-1 P20 antibody (AWA40005) at 1/50 dilution(green ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Cleaved Caspase-1 P20 antibody (AWA40005) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
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Fig : Western blot analysis of Cleaved-Caspase-1 p20 (D210) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA40005, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell Lane 2: NIH3T3 cell Lane 3: Raw264.7 cell Lane 4: A549 cell Lane 5: Hek293 cell Exposure time: 7 min Predicted band size: 25 kDa Observed band size: 20/25 kDa |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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