Beclin 1 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA12678

应用：

WB,IHC-P,IF-C,IF-T

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

52 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

Beclin1; Beclin-1; APG6; ATG 6; ATG6; ATG6 autophagy related 6 homolog;Bcl-2-interacting protein beclin; VPS 30; VPS30; GT197;Protein GT197; Beclin 1 autophagy related; BECN 1; Becn1; Coiled coil myosin like BCL2 interacting protein; Coiled-coil myosin-like BCL2-interacting protein

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC-P 1:50-1:200

IF-C 1:50-1:200

IF-T 1:50-1:200

Immunogen

Information

Gene Name：

BECN1

Protein Name：

Beclin-1

Gene ID：

8678 (Human)

56208 (Mouse)

114558 (Rat)

SwissPro：

Q14457 (Human)

O88597 (Mouse)

Q91XJ1 (Rat)

Subcellular Location：

Cytoplasm. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Endoplasmic reticulum membrane. Mitochondrion membrane. Endosome. Cytoplasmic vesicle, autophagosome. Mitochondrion. Nucleus.

Immunogen：

Recombinant protein within human Beclin 1. AA range: 351-450.

Specificity:

Beclin 1 Monoclonal Antibody detects endogenous levels of Beclin 1 protein.

Product images
	Fig : Western blot analysis of Beclin1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 90 minutes at room temperature. The primary antibody (AWA12678, 1/2000) was used in TBST at room temperature for 90 minutes. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate
	Fig : Western blot analysis of Benclin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA12678, 1/2000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC-12 cell Lane 2: N-2a cell Lane 3: SH-SY5Y cell Lane 4: K-562 cell Lane 5:HELA cell Lane 6:HEK-293 cell
	Fig: Immunocytochemistry analysis of Hela cells labeling Beclin 1with Rabbit anti-Beclin 1 antibody （AWA12678）at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Beclin 1 antibody （AWA12678）at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0003) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue with Rabbit anti-Beclin 1 antibody (AWA12678) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA126782) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-seahorse tissue with Rabbit anti-Beclin 1 antibody (AWA12678) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA126782) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-Beclin 1 antibody (AWA12678) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA126782) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-colon tissue with Rabbit anti-Beclin 1 antibody (AWA12678) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA126782) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-cortex tissue with Rabbit anti-Beclin 1 antibody (AWA12678) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA126782) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-seahorse tissue with Rabbit anti-Beclin 1 antibody (AWA12678) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA126782) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue with Rabbit anti-Beclin 1 antibody (AWA12678) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12678) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Immunohistochemical analysis of paraffin-embedded rat-colon tissue with Rabbit anti-Beclin 1 antibody (AWA12678) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12678) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Immunohistochemical analysis of paraffin-embedded rat-cortex tissue with Rabbit anti-Beclin 1 antibody (AWA12678) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12678) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.