GPX4 Recombinant Rabbit Monoclonal Antibody

货号：

AWA11352

应用：

WB,IHC-P,IF-C,IF-T,FCM

反应性：

Human,Mouse,Rat,Zebrafish,Monkey

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat,Zebrafish,Monkey

Molecular Wt:

Predicted MW: 22 kDa
Observed MW: 22 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1mg/ml

Other Names:

Glutathione peroxidase 4; GPX 4; GPX-4; GPX4; GSHPx-4; MCSP; mitochondrial; PHGPx; Phospholipid hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase; snGPx; Phospholipid hydroperoxide glutathione peroxidase mitochondrial; snPHGPx; Sperm nucleus glutathione peroxidase

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:500-1:5000
IHC-P 1:100-1:1000
IF-C 1:50-1:200
IF-T 1:100-1:500
FCM 1: 10000

Immunogen
Information

Gene Name:

GPX4

Protein Name:

Phospholipid hydroperoxide glutathione peroxidase GPX4

Gene ID:

2879 (Human)
625249 (Mouse)
29328 (Rat)

SwissPro:

P36969 (Human)
O70325 (Mouse)
P36970 (Rat)

Subcellular Location:

Mitochondrion. Cytoplasm.

Immunogen:

Synthetic peptide within human GPX4. AA range: 23-72.

Specificity:

GPX4 Monoclonal Antibody detects endogenous levels of GPX4 protein.

Product images
	Fig : Western blot analysis of GPX4 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA11352, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HCT116 cell Lane 2: U251 cell Lane 3: Jurkat cell Lane 4: N-2a cell Lane 5: Raw264.7 cell Predicted molecular weight:22 kDa Observed molecular weight:22 kDa
	Fig : Western blot analysis of GPX4 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11352, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell Lane 2: Mouse testis Lane 3: Mouse heart Lane 4: Rat testis Predicted molecular weight:22 kDa Observed molecular weight:22 kDa Exposure time：15s
	Fig: Fluorescence immunohistochemical analysis of Rat-cerebellum tissue (Formalin / PFA-fixed paraffin-embedded sections) with rabbit anti-GPX4 antibody (AWA11352 ) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11352) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig: Fluorescence immunohistochemical analysis of Mouse-kidney tissue (Formalin / PFA-fixed paraffin-embedded sections) with rabbit anti-GPX4 antibody (AWA11352 ) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11352) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue with Rabbit anti-GPX4(AWA11352) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11352) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Rabbit anti-GPX4(AWA11352) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11352) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Immunocytochemistry analysis of HeLa cells labeling GPX4 with rabbit anti-GPX4 antibody (AWA11352) at 1/50 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with rabbit anti-GPX4 antibody ( AWA11352) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-RABBIT IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).