xCT/SLC7A11 Recombinant Mouse Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA00502

应用：

WB,IHC-P,IF,FC

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human,Mouse,Rat

Molecular Wt:

55 kDa

Clonality:

Monoclonal

Isotype:

IgG2b

Concentration:

1 mg/ml

Other Names:

CCBR1; Cysteine/glutamate transporter; SLC7A11; solute carrier family 7; xCT; Amino acid transport system xc

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:5000

IHC-P 1:50-1:200

IF 1:50-1:200

FCM 1:500-1:1000

Immunogen

nformation

Gene Name：

SLC7A11

Protein Name：

Cystine/glutamate transporter

Gene ID：

23657 (Human)

26570 (Mouse)

310392 (Rat)

SwissPro：

Q9UPY5 (Human)

Q9WTR6 (Mouse)

Subcellular Location：

Cell membrane. Cell projection, microvillus membrane.

Immunogen：

293 cell line overexpressing SLC7A11.

Specificity:

xCT/SLC7A11 Monoclonal Antibody detects endogenous levels of xCT/SLC7A11 protein.

Product images
	Fig: Immunocytochemistry analysis of A549 cells labeling xCT/SLC7A11 with mouse anti-xCT/SLC7A11 antibody (AWA00502) at 1/50 dilution(red). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with mouse anti-xCT/SLC7A11 antibody (AWA00502) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-mouse IgG H&L (iFluor™ 594, AWS0004) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Immunohistochemical analysis of paraffin-embedded mouse-lung tissue with Mouse anti-xCT/SLC7A11 antibody (AWA00502) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00502) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-lung tissue with Mouse anti-xCT/SLC7A11 antibody (AWA00502) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00502) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.