Vinculin Recombinant Mouse Monoclonal Antibody

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货号：

AWA00533

应用：

WB,IHC-P,IF-C,IF-T,IP

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
50μL ¥580 1-3个工作日
100μL ¥920 1-3个工作日
500μL ¥3800 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human, Mouse, Rat

Molecular Wt:

124 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

1mg/ml

Other Names:

VCL; Metavinculin; CMD1W; MVCL; MV; CMH15; HEL114; Vinculin

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:100000
IHC-P 1:100-1:500
IF-C 1:50-1:500
IF-T 1:100-1:1000
IP 1:100-1:300

Immunogen
Information

Gene Name:

VCL

Protein Name:

Vinculin

Gene ID:

7414 (Human)
22330 (Mouse)
305679 (Rat)

SwissPro:

P18206 (Human)
Q64727 (Mouse)
P85972 (Rat)

Immunogen
Information

Subcellular Location:

Cell membrane. Cell junction, adherens junction. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Cell membrane, sarcolemma. Cell projection, podosome.

Immunogen:

Synthetic peptide within human Vinculin. AA range: 1000-1060.

Specificity:

Vinculin Monoclonal Antibody detects endogenous levels of Vinculin protein.

Product images
	Fig : Western blot analysis of Vinculin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA00533, 1/10000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell Lane 2: HaCaT cell Lane 3: A431 cell Lane 4: A549 cell Lane 5: PC12 cell Lane 6: HepA1-6 cell Lane 7: Mouse heart Predicted molecular weight:124 kDa Observed molecular weight:124 kDa