ZO-1 Rabbit Polyclonal Antibody

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货号:
AWA75003
应用:
IHC-P,IF,FCM
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    195 kDa

    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml

    Other Names:

    Tight junction protein 1; Tight junction protein ZO 1; TJP1; ZO 1; ZO1; ZO-1; Zona occludens protein 1; Zonula occludens protein 1

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

    Purification:

    Affinity-chromatography

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    IHC-P 1:100-1:500 IF 1:50-1:200
    FCM 1:50-1:100

    Immunogen
    Information

    Gene Name:

    TJP1

    Protein Name:

    Tight junction protein ZO-1

    Gene ID:

    7082 (Human)
    21872 (Mouse)
    292994 (Rat)

    SwissPro:

    Q07157 (Human)
    P39447 (Mouse)
    A6JBM5 (Rat)

    Subcellular Location:

    Cell membrane. Cell junction, tight junction. Cell junction. Cell junction, gap junction. Cell projection, podosome.

    Immunogen:

    Synthetic peptide of human ZO-1. AA range: 500-514 and 1682-1696.

    Specificity:

    ZO-1 Polyclonal Antibody detects endogenous levels of ZO-1 protein.

    Product images
    ZO-1 Rabbit Polyclonal Antibody - 1 Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Rabbit anti-ZO-1 antibody (AWA75003) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA75003) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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