GSDMD Rabbit Polyclonal Antibody_艾碧维生物官网

货号：

AWA75002

应用：

WB,IHC-P,IF,FCM

反应性：

Human,Mouse,Rat,Pig

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat,Pig

Molecular Wt:

53 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1mg/ml

Other Names:

Gasdermin-D; C-terminal; Gasdermin-D; gasderminD; Gasdermin domain-containing protein 1; gasdermin D; DF5L; DFNA5L; FKSG10; GSDMDC1; GSDMD

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:500-1:2000
IHC-P 1:100-1:500
IF 1:50-1:200
FCM 1:50-1:100

Immunogen
Information

Gene Name:

GSDMD

Protein Name:

Gasdermin-D

Gene ID:

79792 (Human)
69146 (Mouse)

SwissPro:

P57764 (Human)
Q9D8T2 (Mouse)

Subcellular Location:

Cytoplasm, cytosol. Inflammasome. Cell membrane. Secreted. Mitochondrion membrane.

Immunogen:

Synthetic peptide of human GSDMD. AA range: 56-70.

Specificity:

GSDMD Polyclonal Antibody detects endogenous levels of GSDMD protein.

Product images
	Fig: Immunocytochemistry analysis of PC-3 cells labeling GSDMD with Rabbit anti-GSDMD antibody (AWA75002) at 1/50 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-GSDMD antibody (AWA75002) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Western blot analysis of GSDMD on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA75002, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC12 cell Lane 2: SIHa cell Lane 3: CAL27 cell Lane 4: Rat stomach Lane 5: CAKI-1 cell Lane 6: THP-1 cell Lane 7: 3D4/21 cell Lane 8: A431 cell Lane 9: HT1080 cell Predicted molecular weight:53 kDa Observed molecular weight:53 kDa(GSDMD)；35KD(GSDMD N-Terminal)； Exposure time：3 min
	Fig：Flow cytometric analysis of RAW264.7 cells labeling GSDMD. Overlay histogram showing RAW264.7 cells stained with GSDMD (green line). The cells were fixed in 4% paraformaldehyde for 30 minutes at 37 ℃, permeabilized with 0.02% Triton X-100 in PBS for 30 minutes,and then stained with the primary antibody(AWA75002, 1µg/1x106 cells) for 30 min at 4°C. The secondary antibody used was an Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody (AWS0005b) at 1/500 dilution for 30 min at 4ºC. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Rabbit anti-GSDMD antibody (AWA75002) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA75002) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.