IL-1β Rabbit Polyclonal Antibody

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货号:
AWA75001
应用:
WB,FCM
反应性:
Human,Mouse,Rat,Pig
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat,Pig

    Molecular Wt:

    Predicted MW: 31 kDa
    Observed MW: 37 kDa

    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml

    Other Names:

    Il-1b; IL-1beta; IL1β; IL-1β

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

    Purification:

    Affinity-chromatography

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    WB 1:1000-1:5000
    FCM 1:50-1:100

    Immunogen
    Information

    Gene Name:

    IL1B

    Protein Name:

    Interleukin-1 beta

    Gene ID:

    3553 (Human)
    16176 (Mouse)

    SwissPro:

    P01584 (Human)
    P10749 (Mouse)
    Q63264 (Rat)

    Subcellular Location:

    Cytoplasm, cytosol. Secreted. Lysosome. Secreted, extracellular exosome.

    Immunogen:

    Synthetic peptide of human IL-1β. AA range: 206-220.

    Specificity:

    IL-1β Polyclonal Antibody detects endogenous levels of IL-1β protein.

    Product images
    IL-1β Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of IL-1β on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA75001, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: LLC cell
    Lane 2: THP-1 cell treated with 80 nM TPA overnight and then 100 ng/ml LPS for 6 h and 300 ng/ml Brefeldin A for the last 3 h
    Lane 3: 5637 cell
    Lane 4: RAW264.7 cell
    Lane 5: J774A.1 cell
    Lane 6: 3D4/21 cell
    Lane 7: A549 cell
    Lane 8: HSC-T6 cell
    Lane 9: A431 cell

    Predicted molecular weight:31 kDa
    Observed molecular weight:37 kDa
    Exposure time:3 min
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