KiSS-1R Rabbit Polyclonal Antibody

货号：

AWA58031

应用：

WB,IHC-P

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 43 kDa
Observed MW: 50-72 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1mg/ml

Other Names:

G-protein coupled receptor OT7T175; G-protein coupled receptor 54; Hypogonadotropin 1; KISS 1 receptor; Kisspeptins receptor; GPR54; HH8; CPPB1; AXOR 12; AXOR12; HOT7T175; KISS1R; KISS 1R; KISS-1R

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:500

Immunogen
Information

Gene Name:

KISS1R

Protein Name:

KiSS-1 receptor

Gene ID:

84634 (Human)
114229 (Mouse)
78976 (Rat)

SwissPro:

Q969F8 (Human)
Q91V45 (Mouse)
Q924U1 (Rat)

Immunogen
Information

Subcellular Location:

Cell membrane.

Immunogen:

Synthetic peptide derived from human KiSS-1R. AA range: 121-200.

Specificity:

KiSS-1R Polyclonal Antibody detects endogenous levels of KiSS-1R protein.

Product images
	Fig : Western blot analysis of KISS-1R on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA58031 , 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell Lane 2: HeLa cell Lane 3: SH-SY5Y cell Lane 4: NIH3T3 cell Lane 5: A549 cell Predicted molecular weight:43 kDa Observed molecular weight:~70 kDa （glycosylation）

	Fig : Western blot analysis of KiSS-1R on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA58031, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell Predicted molecular weight:43 kDa Observed molecular weight:43 kDa~170kDa （glycosylation）

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-KiSS-1R antibody (AWA58031) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA58031) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-lung tissue with Rabbit anti-KiSS-1R antibody (AWA58031) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA58031) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.