CD8 alpha Recombinant Rabbit Monoclonal Antibody

货号：

AWA22029

应用：

WB,IHC-P,IHC-F,IP,FCM

反应性：

Mouse

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Mouse

Molecular Wt:

26 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1mg/ml

Other Names:

CD8 antigen; CD8A molecule; CD8 antigen alpha polypeptide; Cd8a; CD8; Leu2; MAL; p32; Ly 2; Ly-2; Ly 35; Ly-35; Ly B; Ly-B; Lyt 2; Lyt-2; CD8A; CD8 alpha

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IHC-F 1:100-1:1000
IP 0.5-4.0 ug for 0.5-3.0 mg of total protein lysate.
FCM 1:50-1:200

Immunogen
Information

Gene Name:

CD8A

Protein Name:

T-cell surface glycoprotein CD8 alpha chain

Gene ID:

925 (Human)
12525 (Mouse)
24930 (Rat)

SwissPro:

P01732 (Human)
P01731 (Mouse)
P07725 (Rat)

Immunogen
Information

Subcellular Location:

Cell membrane. Secreted.

Immunogen:

Recombinant protein within human CD8 alpha.

Specificity:

CD8 alpha Monoclonal Antibody detects endogenous levels of CD8 alpha protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti- CD8A antibody (AWA22029) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA22029) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Western blot analysis of CD8 alpha on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA22029, 1/4000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen Predicted molecular weight:26 kDa Observed molecular weight:30~40 kDa