MFF Recombinant Rabbit Monoclonal Antibody

货号：

AWA22017

应用：

WB,IHC-P,IF,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 38 kDa
Observed MW: 38 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

0.841mg/ml

Other Names:

C2orf33; mitochondrial fission factor; EMPF2; GL004; MFF

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IF 1:100-1:800
FCM 1:50-1:200

Immunogen
Information

Gene Name:

MFF

Protein Name:

Mitochondrial fission factor

Gene ID:

56947 (Human)
75734 (Mouse)
301563 (Rat)

SwissPro:

Q9GZY8 (Human)
Q6PCP5 (Mouse)
Q4KM98 (Rat)

Immunogen
Information

Subcellular Location:

Mitochondrion outer membrane. Peroxisome. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle.

Immunogen:

Fusion protein amino acids 1-173 (cytoplasmic N-terminal exons 1, 2, 3 and 4) and 272-322 (cytoplasmic N-terminal exons 8 and most of 9) of mostly human MFF.

Specificity:

MFF Monoclonal Antibody detects endogenous levels of MFF protein.

Product images
	Fig : Western blot analysis of MFF on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA22017, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell Lane 2: SIHa cell Predicted molecular weight:38 kDa Observed molecular weight:38 kDa

	Fig : Western blot analysis of MFF on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA22017, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: DU145 cell Lane 2: Mouse brain Predicted molecular weight:38 kDa Observed molecular weight:38 kDa

	Fig: Immunocytochemistry analysis of DU145 cells labeling MFF with Rabbit anti-MFF antibody (AWA22017) at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.5% Triton X-100 in PBS for 15 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-MFF antibody (AWA22017) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).