VGLUT1 Recombinant Rabbit Monoclonal Antibody

货号：

AWA22014

应用：

WB,IHC-P,IHC-F,IF-C,IF-T,mIHC,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 62 kDa
Observed MW: 62 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1.148mg/ml

Other Names:

Brain-specific Na(+)-dependent inorganic phosphate cotransporter; BNPI; SLC17A7; VGLUT1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IHC-F 1:100-1:500
IF-C 1:50-1:500
IF-T 1:100-1:500
mIHC 1:100-1:1000
IP 1:100-1:300

Immunogen
Information

Gene Name:

SLC17A7

Protein Name:

Vesicular glutamate transporter 1

Gene ID:

57030 (Human)
72961 (Mouse)
116638 (Rat)

SwissPro:

Q9P2U7 (Human)
Q3TXX4 (Mouse)
Q62634 (Rat)

Immunogen
Information

Subcellular Location:

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell membrane. Synapse, synaptosome.

Immunogen:

Synthetic peptide of human VGLUT1.

Specificity:

VGLUT1 Monoclonal Antibody detects endogenous levels of VGLUT1 protein.

Product images
	Fig: Multiplex immunohistochemistry analysis of Mouse-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). Merged staining of anti-VGLUT1 (AWA22014; green; TSA-520:AWI0688), anti-DCX (AWA13631; red; TSA-570: AWI0689).Antibody Wash Solution (for mIHC) (AWI0707, 37℃, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.The section was incubated in three rounds of staining; in the order of VGLUT1 (AWA22014) (1/200 dilution), anti-DCX (AWA13631) (1/200 dilution); each using a separate fluorescent tyramide signal amplification system. DAPI (blue, AWC0291) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual TSA dyes was performed using a pannoramic midi. This image was generated from the hybridoma version.

	Fig : Western blot analysis of VGLUT1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA22014, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse brain Lane 2: Mouse brain(unboiled) Predicted molecular weight:62 kDa Observed molecular weight:62 kDa

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti- VGLUT1 antibody (AWA22014) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA22014) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig: Immunocytochemistry analysis of SH-SY5Y cells labeling VGLUT1 with Rabbit anti-VGLUT1 antibody (AWA22014) at 1/300 dilution(Green ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-VGLUT1 antibody (AWA22014) at 1/300 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).