ISG15 Recombinant Rabbit Monoclonal Antibody

货号：

AWA22012

应用：

WB,IHC-P,IF-C,FCM

反应性：

Human,Mouse,Rat,Cat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Mouse, Rat, Cat

Molecular Wt:

Predicted MW: 18 kDa
Observed MW: 15-17 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1.125mg/ml

Other Names:

G1P2; hUCRP; IFI15; IP17; ISG15 ubiquitin like modifier; Ubiquitin like protein ISG15; UCRP; IMD38; ISG15

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:500
IF-C 1:50-1:500
FCM 1:50-1:200

Immunogen
Information

Gene Name:

ISG15 G1P2 UCRP

Protein Name:

Ubiquitin-like protein ISG15

Gene ID:

9636 (Human)
100038882 (Mouse)
298693 (Rat)

SwissPro:

P05161 (Human)
Q64339 (Mouse)
D4A3X3 (Rat)

Immunogen
Information

Subcellular Location:

Cytoplasm. Secreted.

Immunogen:

Recombinant fragment of human ISG15.

Specificity:

ISG15 Monoclonal Antibody detects endogenous levels of ISG15 protein.

Product images
	Fig : Western blot analysis of ISG15 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA22012, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Daudi cell Lane 2: A204 cell Lane 3: Hela cell Lane 4: PC3 cell Lane 5: A431 cell Lane 6: HepG2 cell Lane 7: MDA-MB-231 cell Predicted molecular weight:18 kDa Observed molecular weight:15-17 kDa

	Fig: Immunocytochemistry analysis of RAW264.7 cells（treated with 1 µg/mL LPS for 24h and then treated with 1 µg/mL Brefeldin A for 3h） labeling ISG15 with Rabbit anti-ISG15 antibody（AWA22012）at 1/50 dilution(Green ). Cells were fixed in 100% Methanol（-20℃ precool） for 20 minutes , and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-ISG15 antibody （AWA22012）at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig：Flow cytometric analysis of HELA cells labeling ISG15. Overlay histogram showing HELA cells stained with ISG15 (green line). The cell were fixed in 4% paraformaldehyde for 30 minutes at 37 ℃, permeabilized with 0.02% Triton X-100 in PBS for 30 minutes,and then stained with the primary antibody(AWA22012, 1:50) for 30 min at 4°C. The secondary antibody used was an Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody (AWS0005b) at 1/500 dilution for 30 min at 4ºC. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti- ISG15 antibody (AWA22012) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA22012) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.