ISG15 Recombinant Rabbit Monoclonal Antibody

货号：

AWA22012

应用：

WB,IHC-P,IF-C,FCM

反应性：

Human,Mouse,Rat,Cat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat,Cat

Molecular Wt:

Predicted MW: 18 kDa
Observed MW: 15-17 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1.125mg/ml

Other Names:

G1P2; hUCRP; IFI15; IP17; ISG15 ubiquitin like modifier; Ubiquitin like protein ISG15; UCRP; IMD38; ISG15

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:500-1:2000
IHC-P 1:100-1:500
IF-C 1:50-1:200
FCM 1:50-1:200

Immunogen
Information

Gene Name:

ISG15 G1P2 UCRP

Protein Name:

Ubiquitin-like protein ISG15

Gene ID:

9636 (Human)
100038882 (Mouse)
298693 (Rat)

SwissPro:

P05161 (Human)
Q64339 (Mouse)
D4A3X3 (Rat)

Immunogen
Information

Subcellular Location:

Cytoplasm. Secreted.

Immunogen:

Recombinant fragment of human ISG15.

Specificity:

ISG15 Monoclonal Antibody detects endogenous levels of ISG15 protein.

Product images
	Fig : Western blot analysis of ISG15 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA22012, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Daudi cell Lane 2: A204 cell Lane 3: Hela cell Lane 4: PC3 cell Lane 5: A431 cell Lane 6: HepG2 cell Lane 7: MDA-MB-231 cell Predicted molecular weight:18 kDa Observed molecular weight:15-17 kDa
	Fig：Flow cytometric analysis of HELA cells labeling ISG15. Overlay histogram showing HELA cells stained with ISG15 (green line). The cell were fixed in 4% paraformaldehyde for 30 minutes at 37 ℃, permeabilized with 0.02% Triton X-100 in PBS for 30 minutes,and then stained with the primary antibody(AWA22012, 1:50) for 30 min at 4°C. The secondary antibody used was an Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody (AWS0005b) at 1/500 dilution for 30 min at 4ºC. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).