NMDAR2B/GRIN2B Recombinant Rabbit Monoclonal Antibody

货号：

AWA22009

应用：

WB,IHC-P,IF

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 166 kDa
Observed MW: 166 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

0.811mg/ml

Other Names:

GluN2B; GRIN2B; hNR3; NMDAR2B; NR2B; NR3; MRD6; DEE27; EIEE27; NMDAR2B; NMDAR2B/GRIN2B

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IF 1:100-1:800

Immunogen
Information

Gene Name:

GRIN2B

Protein Name:

Glutamate receptor ionotropic, NMDA 2B

Gene ID:

2904 (Human)
14812 (Mouse)
24410 (Rat)

SwissPro:

Q13224 (Human)
Q01097 (Mouse)
Q00960 (Rat)

Immunogen
Information

Subcellular Location:

Cell membrane. Postsynaptic cell membrane. Cell projection, dendrite. Late endosome. Lysosome. Cytoplasm, cytoskeleton.

Immunogen:

Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B (accession number Q00960) produced recombinantly in E. Coli.

Specificity:

NMDAR2B/GRIN2B Monoclonal Antibody detects endogenous levels of NMDAR2B protein.

Product images
	Fig : Western blot analysis of NMDAR2B on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA22009, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse brain Lane 2: Rat brain Predicted molecular weight:166 kDa Observed molecular weight:166 kDa

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-cerebellum tissue with Rabbit anti- NMDAR2B antibody (AWA22009) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA22009) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig: Immunocytochemistry analysis of SH-SY5Y cells labeling NMDAR2B with Rabbit anti-NMDAR2B antibody（AWA22009）at 1/250 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-NMDAR2B antibody （AWA22009）at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).