HIF-1α Recombinant Rabbit Monoclonal Antibody

货号：

AWA14300

应用：

WB,IHC-P,IF-C

反应性：

Human,Mouse

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Mouse

Molecular Wt:

Predicted MW: 93 kDa
Observed MW: 120 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1mg/ml

Other Names:

ARNT interacting protein; bHLHe78; HIF 1 alpha; HIF 1alpha; HIF1; HIF-1; HIF1 ALPHA; HIF-1 alpha; HIF1A; Member of PAS protein 1; MOP1; PASD8; HIF-1α

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IF-C 1:100-1:500

Immunogen
Information

Gene Name:

HIF1A

Protein Name:

Hypoxia-inducible factor 1-alpha

Gene ID:

3091 (Human)
15251 (Mouse)

SwissPro:

Q16665 (Human)
Q61221 (Mouse)

Immunogen
Information

Subcellular Location:

Cytoplasm. Nucleus. Nucleus speckle.

Immunogen:

Synthetic peptide within human HIF-1α. AA range: 441-490.

Specificity:

HIF-1α Monoclonal Antibody detects endogenous levels of HIF-1α protein.

Product images
	Fig : Western blot analysis of HIF-1α on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA14300, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NRK-49F cell Predicted molecular weight:93 kDa Observed molecular weight:120 kDa

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue with Rabbit anti-HIF-1α antibody (AWA14300) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA14300) at 1/100 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.