Ferritin heavy chain Recombinant Rabbit Monoclonal Antibody

货号：

AWA12692

应用：

WB,IHC-P,IF-C,IF-T

反应性：

Human,Mouse,Rat,Zebrafish,Monkey,Pig

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Mouse, Rat, Zebrafish, Monkey, Pig

Molecular Wt:

Predicted MW: 21 kDa
Observed MW: 21 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1.057mg/ml

Other Names:

Cell proliferation-inducing gene 15 protein; Ferritin H subunit; Ferritin heavy chain; Ferritin heavy polypeptide 1; Ferritin L subunit; Ferritin, heavy polypeptide; FTH; FTH1; FTL

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:50-1:1000
IF-C 1:100-1:800
IF-T 1:100-1:1000

Immunogen
Information

Gene Name:

FTH1

Protein Name:

Ferritin heavy chain

Gene ID:

2495 (Human)
14319 (Mouse)
25319 (Rat)

SwissPro:

P02794 (Human)
P09528 (Mouse)
P19132 (Rat)

Immunogen
Information

Subcellular Location:

Cytoplasm. Lysosome. Cytoplasmic vesicle, autophagosome.

Immunogen:

Synthetic peptide within human Ferritin heavy chain. AA range:range: 58-99.

Specificity:

Ferritin heavy chain Monoclonal Antibody detects endogenous levels of Ferritin heavy chain protein.

Product images
	Fig: Multiplex immunohistochemistry analysis of RAT-Skin tissue (Formalin/PFA-fixed paraffin-embedded sections). Merged staining of anti-VEGFA (AWA11349; green; TSA-520:AWI0688), anti-Ferritin (AWA12692; red; TSA-570: AWI0689), anti-CD36 (AWA13657; white; TSA-690: AWI0691), anti-S100A4 (AWA11327; orange; TSA-620: AWI0690). Antibody Wash Solution (for mIHC) (AWI0707, 37℃, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. The section was incubated in three rounds of staining; in the order of VEGFA (AWA11349) (1/200 dilution), anti-Ferritin (AWA12692) (1/200 dilution), anti-CD36 (AWA13657) (1/200 dilution), anti-S100A4 (AWA11327) (1/200 dilution); each using a separate fluorescent tyramide signal amplification system. DAPI (blue, AWC0291) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual TSA dyes was performed using a pannoramic midi. This image was generated from the hybridoma version.

	Fig : Western blot analysis of Ferritin heavy chain on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12692, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HuH-7 cell Lane 2: Mouse liver Lane 3: Rat liver Lane 4: Rat brain Lane 5: Zebrafish Predicted molecular weight:21 kDa Observed molecular weight:21 kDa

	Fig: Fluorescence immunohistochemical analysis of Rat-ovary tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Ferritin heavy chain antibody (AWA12692) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12692) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig :Western blot analysis of Ferritin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12692, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HeLa cell Lane 2: MCF-7 cell Lane 3: HEK293 cell Lane 4: HEPA1-6 cell Lane 5: A549 cell Predicted molecular weight: 21kDa Observed molecular weight: 21kDa

	Fig : Western blot analysis of Ferritin heavy chain on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12692, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell Lane 2: Raw264.7 cell Lane 3: HSC-T6 cell Lane 4: COS7 cell Predicted molecular weight:21 kDa Observed molecular weight:21 kDa

	Fig: Immunocytochemistry analysis of Raw264.7 cells labeling Ferritin heavy chain with Rabbit anti-Ferritin heavy chain antibody （AWA12692）at 1/150 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Ferritin heavy chain antibody （AWA12692 ）at 1/150 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig: Fluorescence immunohistochemical analysis of Mouse-testicle tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Ferritin heavy chain antibody (AWA12692) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12692) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig: Immunocytochemistry analysis of Hela cells labeling Ferritin heavy chain with Rabbit anti-Ferritin heavy chain antibody （AWA12692）at 1/150 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Ferritin heavy chain antibody （AWA12692）at 1/150 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig: Fluorescence immunohistochemical analysis of Mouse-stomach tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Ferritin heavy chain antibody (AWA12692) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12692) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig: Fluorescence immunohistochemical analysis of Rat-spleen tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-Ferritin heavy chain antibody (AWA12692) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12692) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig: Immunocytochemistry analysis of Hela cells labeling Ferritin heavy chain with Rabbit anti-Ferritin heavy chain antibody (AWA12692) at 1/150 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Ferritin heavy chain antibody (AWA12692) at 1/150 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig: Fluorescence immunohistochemical analysis of Mouse-colon tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Ferritin heavy chain antibody (AWA12692) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12692) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.