CD11b Recombinant Rabbit Monoclonal Antibody

货号：

AWA10296

应用：

WB,IHC-P,mIHC

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 127 kDa
Observed MW: 170 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1mg/ml

Other Names:

CD11b/CD18; CD11B/Integrin Alpha M; CR 3 alpha chain; CR-3 alpha chain; CR3; CR3A; Integrin alpha M; Integrin alpha M chain; ITGAM; MAC 1; MAC1A; Mac-1a; MO1A; Neutrophil adherence receptor; SLEB6; Ly-40; CD11B; CD11b

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
mIHC 1:100-1:1000

Immunogen
Information

Gene Name:

ITGAM

Protein Name:

Integrin alpha-M

Gene ID:

3684 (Human)
16409 (Mouse)

SwissPro:

P11215 (Human)
E9Q604 (Mouse)

Immunogen
Information

Subcellular Location:

Cell membrane. Membrane raft.

Immunogen:

Recombinant protein within human CD11b.

Specificity:

CD11b Monoclonal Antibody detects endogenous levels of CD11b protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-lung tissue with Rabbit anti-CD11b (AWA10296) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10296) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded RM-1 cell derived xenograft with Rabbit anti-CD11b (AWA10296) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10296) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Western blot analysis of CD11b on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10296, 1/8000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse bone marrow cell Lane 2: J774A.1 cell Predicted molecular weight:127 kDa Observed molecular weight:~160 kDa