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COX1 Recombinant Mouse Monoclonal Antibody [M48A02]

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货号:
AWA06074
应用:
WB,IHC-P,IP,FCM
反应性:
Human,Mouse,Rat
来源:
Mouse
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Mouse

    Reactivity:

    Human, Mouse, Rat

    Molecular Wt:

    69 kDa

     

    Clonality:

    Monoclonal

    Isotype:

    IgG2a

    Concentration:

    1.125mg/ml

     

    Other Names:

    COX-1/Cyclooxygenase-1; COX3; COX 3; Cox3; Cyclooxygenase 1; PCOX1; PGG/HS; PGH synthase 1; PGHS 1; PGHS-1; PGHS1; PHS 1; PHS1; Prostaglandin G/H synthase 1; Prostaglandin H2 synthase 1; PTGHS; PTGS1; Prostaglandin-endoperoxide synthase 1; COX-1; COX 1; COX1

     

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

     

    Purification:

    Affinity-chromatography

     

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    WB 1:1000-1:5000
    IHC-P 1:100-1:1000
    IP 0.5-4.0 ug for 0.5-3.0 mg of total protein lysate.
    FCM 1:50-1:200

    Immunogen
    Information

    Gene Name:

    PTGS1

    Protein Name:

    Prostaglandin G/H synthase 1

     

    Gene ID:

    5742 (Human)  
    19224 (Mouse)

    SwissPro:

    P23219 (Human)  
    P22437 (Mouse)  
    Q63921 (Rat)

    Immunogen
    Information

    Subcellular Location:

    Microsome membrane. Endoplasmic reticulum membrane.

     

    Immunogen:

    Synthetic peptide within human COX1. AA range: 116-159.

     

    Specificity:

    COX1 Monoclonal Antibody detects endogenous levels of COX1 protein.


    Product images
    COX1 Recombinant Mouse Monoclonal Antibody [M48A02] - 1 Fig : Western blot analysis of COX1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06074, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: NIH3T3 cell
    Lane 2: Hela cell
    Lane 3: SCC25 cell
    Predicted molecular weight:69 kDa
    Observed molecular weight:65~72 kDa
    COX1 Recombinant Mouse Monoclonal Antibody [M48A02] - 2 Fig : Western blot analysis of COX1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06074, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Raw264.7 cell
    Lane 2: A431 cell
    Lane 3: C2C12 cell
    Lane 4: L6 cell
    Predicted molecular weight:69 kDa
    Observed molecular weight:65~72 kDa

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