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c-Jun(Phospho Ser63) Recombinant Mouse Monoclonal Antibody [M43D08]

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货号:
AWA06072
应用:
WB,IHC-P,IF-C
反应性:
Human,Mouse,Rat
来源:
Mouse
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Mouse

    Reactivity:

    Human, Mouse, Rat

    Molecular Wt:

    36 kDa

     

    Clonality:

    Monoclonal

    Isotype:

    IgG2a

    Concentration:

    0.578mg/ml

     

    Other Names:

    Activator protein 1; AP 1; AP1; Enhancer Binding Protein AP1; Jun Activation Domain Binding Protein; JUN; Jun oncogene; JUN protein; Jun proto oncogene; JUNC; Oncogene JUN; p39; Proto oncogene c jun; Proto oncogene cJun; Proto-oncogene c-jun; Transcription Factor AP 1; Transcription factor AP-1; cJun; c-Jun; c-Jun(Phospho Ser63)

     

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

     

    Purification:

    Affinity-chromatography

     

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    WB 1:1000-1:5000
    IHC-P 1:100-1:1000
    IF-C 1:100-1:800

    Immunogen
    Information

    Gene Name:

    Jun

    Protein Name:

    Transcription factor Jun

     

    Gene ID:

    3725 (Human)
    16476 (Mouse)
    24516 (Rat)

    SwissPro:

    P05412 (Human)
    P05627 (Mouse)
    P17325 (Rat)

    Immunogen
    Information

    Subcellular Location:

    Nucleus.

     

    Immunogen:

    Synthetic phospho-peptide corresponding to residues surrounding Ser63 of human c-Jun. AA range: 31-80.

     

    Specificity:

    Phospho c-Jun(Ser63) Monoclonal Antibody detects endogenous levels of Phospho c-Jun(Ser63) protein.


    Product images
    c-Jun(Phospho Ser63) Recombinant Mouse Monoclonal Antibody [M43D08] - 1 Fig : Western blot analysis of c-Jun(Phospho Ser63) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06072, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: GL261 cell
    Lane 2: HepA1-6 cell
    Lane 3: NIH3T3 cell
    Lane 4: RSC96 cell
    Lane 5: HSC-T6 cell
    Lane 6: PC12 cell
    Predicted molecular weight:36 kDa
    Observed molecular weight:45-50 kDa

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