YAP1(Phospho Ser127) Recombinant Mouse Monoclonal Antibody

货号：

AWA06065

应用：

WB,IHC-P,IF-C,IF-T

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human, Mouse, Rat

Molecular Wt:

54 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

1.002mg/ml

Other Names:

YAP65; Yorkie homolog; 65 kDa Yes-associated protein; YAP 65; YAP65; Yes associated protein 1; Yes associated protein 2; YKI; YAP; YAP1; YAP1(Phospho Ser127)

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IF-C 1:100-1:800
IF-T 1:100-1:1000

Immunogen
Information

Gene Name:

YAP1

Protein Name:

Transcriptional coactivator YAP1

Gene ID:

10413 (Human)
22601 (Mouse)
363014 (Rat)

SwissPro:

P46937 (Human)
P46938 (Mouse)
Q2EJA0 (Rat)

Immunogen
Information

Subcellular Location:

Cytoplasm. Nucleus. Cell junction, tight junction. Cell membrane.

Immunogen:

Synthetic phospho-peptide corresponding to residues surrounding Ser127 of human YAP1.

Specificity:

Phospho YAP1 (Ser127) Monoclonal Antibody detects endogenous levels of Phospho YAP1 (Ser127) protein.

Product images
	Fig : Western blot analysis of YAP1(Phospho Ser127) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06065, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepA1-6 cell Predicted molecular weight:54 kDa Observed molecular weight:~72 kDa

	Fig : Western blot analysis of YAP1(Phospho Ser127) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06065, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell Lane 2: A431 cell Predicted molecular weight:54 kDa Observed molecular weight:~72 kDa