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GANAB Recombinant Mouse Monoclonal Antibody [M48A03]

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货号:
AWA06049
应用:
WB,IHC-P,IF-P,IP
反应性:
Human,Mouse,Rat
来源:
Mouse
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Mouse

    Reactivity:

    Human, Mouse, Rat

    Molecular Wt:

    107 kDa

     

    Clonality:

    Monoclonal

    Isotype:

    IgG2a

    Concentration:

    0.591mg/ml

     

    Other Names:

    G2AN; KIAA0088; Neutral alpha-glucosidase AB; Neutral alpha glucosidase AB; Alpha-glucosidase 2; Glucosidase II subunit alphak; Glucosidase II alpha; Glu II; GluII; GANAB

     

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

     

    Purification:

    Affinity-chromatography

     

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    WB 1:1000-1:5000
    IHC-P 1:100-1:1000
    IF-P 1:100-1:1000
    IP 0.5-4.0 ug for 0.5-3.0 mg of total protein lysate.

    Immunogen
    Information

    Gene Name:

    GANAB

    Protein Name:

    Neutral alpha-glucosidase AB

     

    Gene ID:

    23193 (Human)    
    14376 (Mouse)    
    293721 (Rat)

    SwissPro:

    Q14697 (Human)    
    Q8BHN3 (Mouse)    
    D3ZAN3 (Rat)

    Immunogen
    Information

    Subcellular Location:

    Endoplasmic reticulum. Golgi apparatus. Melanosome.

     

    Immunogen:

    Recombinant protein within human GANAB. AA range: 29-450.

     

    Specificity:

    GANAB Monoclonal Antibody detects endogenous levels of GANAB protein.


    Product images
    GANAB Recombinant Mouse Monoclonal Antibody [M48A03] - 1 Fig : Western blot analysis of GANAB on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06049, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: C6 cell
    Lane 2: EL-4-B5 cell
    Lane 3: MCF-7 cell
    Lane 4: SH-SY5Y cell
    Lane 5: 3D4/21 cell
    Lane 6: Mouse testis
    Lane 7: U87-mg cell
    Lane 8: Mouse brain
    Lane 9: Rat brian
    Predicted molecular weight:107 kDa
    Observed molecular weight:107 kDa
    GANAB Recombinant Mouse Monoclonal Antibody [M48A03] - 2 Fig : Western blot analysis of GANAB on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06049, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: THP-1cell
    Lane 2: A431 cell
    Predicted molecular weight:107 kDa
    Observed molecular weight:107 kDa

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